Restriction Enzymes
New England Biolabs, Inc. BlpI – 500 units
A restriction endonuclease that recognizes the sequence GC^TNA_GC.
New England Biolabs, Inc. Endo H – 10000 units
Endoglycosidase H is a recombinant glycosidase which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins.
New England Biolabs, Inc. HaeIII – 3000 units
A restriction endonuclease that recognizes the sequence GG_^CC.
New England Biolabs, Inc. SalI-HF – 2000 units
A high fidelity restriction endonuclease that recognizes the sequence G^TCGAC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. EagI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GGCC_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. PspOMI – 7500 units
A restriction endonuclease that recognizes the sequence G^GGCC_C.
New England Biolabs, Inc. Nb.BsmI – 1000 units
A restriction endonuclease that recognizes the sequence GAATG_CN.
New England Biolabs, Inc. EcoNI – 5000 units
A restriction endonuclease that recognizes the sequence CCTNN^N_NNAGG.
New England Biolabs, Inc. EcoRI-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. PvuII-HF – 25000 units
A high fidelity restriction endonuclease that recognizes the sequence CAG^CTG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. KasI – 250 units
A restriction endonuclease that recognizes the sequence G^GCGC_C.
New England Biolabs, Inc. DpnII – 1000 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. XcmI – 5000 units
A restriction endonuclease that recognizes the sequence CCANNNN_N^NNNNTGG.
New England Biolabs, Inc. PmeI – 2500 units
A restriction endonuclease that recognizes the sequence GTTT_^AAAC.
New England Biolabs, Inc. BstAPI – 1000 units
A restriction endonuclease that recognizes the sequence GCAN_NNN^NTGC.